Journal: Journal of Biomechanical Engineering
Article Title: A Modified Parallel Plate Flow Chamber to Study Local Endothelial Response to Recirculating Disturbed Flow
doi: 10.1115/1.4044899
Figure Lengend Snippet: Disturbed flow parallel plate flow chamber gasket design and computational fluid dynamics analysis. (a) standard laminar flow (LFG, left) and new disturbed flow (DFG, middle and right) parallel plate flow chamber gaskets, (b) parallel plate chamber assembly, with gasket forming the flow region over endothelial cells cultured on a microscope slide. (c) Time-averaged computational fluid dynamics DFG gasket simulation using RAN κ–ω turbulence with no-slip walls in comsol multiphysics Software. Fluid was cell culture medium (μ = 0.78 × 10−3 Pa·s, 37 °C) and flow rate was 4.24 mL/min; (top) streamlines, (middle) shear stress heat map, (bottom) binary color map indicating shear stress above (red) and below (green) 12 dynes/cm2 (atheroprotective threshold); (d) selected DFG regions of interest, including: DFG-High (shear stress > 20 dynes/cm2); DFG-2 Pa (shear stress ∼20 dynes/cm2); DFG-Recirc (recirculating eddy); and DFG-Low (shear stress < 12 dynes/cm2).
Article Snippet: Steady, nonpulsatile fluid flow simulation was performed in a rectangular 10 × 58 × 0.127 mm 3 3D space ( x , y , z ) with comsol multiphysics v4.0.
Techniques: Cell Culture, Microscopy, Software, Shear